A Practical Device for the Rapid Quantitative Determination of Plant Pigments.

نویسنده

  • W A Beck
چکیده

Solutions of these resins were also used in making permanent mounts of moulds. The slide was first covered with a uniform layer of the resin in precisely the same manner as described above. The film was then air dried for two to three minutes, at the end of which time it still presented a slightly sticky surface. This surface was then impregnated with the fungus in one of two ways. Either the resin side of the slide was laid gently on the colony, removed and air dried, or a portion of the colony was "fished out" with a platinum loop and these fragments placed on the partially solidified resinous layer, allowing the latter to air dry. Then, employing the methods of the mycologist, the organism was fixed, using any solution which has as a solvent, water, e.g., mercuric chloride-formaldehyde solution. A fixing agent having an organic solvent can not be used because of its effect upon the resin. The preparation was then stained, applying any of the dyes used in aqueous solution, such as safranin, erythrosin or fuchsin. It is believed that the application of the synthetic resin is superior to the use of Canada balsam from the standpoint of ease of manipulation, simplicity, rapidity and cost. B. F. SKILES C. E. GEORGI UNIVERSITY OF NEBRASKA

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عنوان ژورنال:
  • Science

دوره 85 2206  شماره 

صفحات  -

تاریخ انتشار 1937